Microbial contamination of toothbrushes among smokers and non-smokers

Document Type : Original Article

Authors

1 Abbasi Shaheed Hospital, Karachi Medical and Dental College, Karachi, Pakistan

2 Professor, Department of Oral Pathology, Karachi Medical and Dental College, Karachi, Pakistan

3 Associate Professor, Department of Pathology, Karachi Medical and Dental College, Karachi, Pakistan

4 United Medical and Dental College, Karachi, Pakistan

5 Associate Professor, Sir Syed College of Medical Sciences for Girls, Karachi, Pakistan

Abstract

BACKGROUND AND AIM: The most common oral hygiene aid used for the maintenance of oral health is the toothbrush and its hygiene is frequently neglected. Cigarette smoking is associated with many oral and periodontal diseases. Its effect can never be neglected on the microbiota of the oral cavity. Therefore, this study was planned to evaluate the microbial contamination of toothbrushes and its relation with smoking. This study aimed to assess the microbial contamination of capped and uncapped toothbrushes of adult smoker and non-smoker men.
METHODS: This randomized study included 50 different used toothbrushes of healthy men, non-smoker and smoker, who may use capped and uncapped toothbrushes. This study was conducted at Karachi Medical and Dental College, Karachi, Pakistan, from September to October 2019. All toothbrushes were evaluated for microbial analysis. Samples were allocated through simple random sampling. Every toothbrush head was put in a sterile container with 10 ml brain heart agar (Oxoid) and incubated in 37 ºC for 24 hours; after which, they were placed in different bases (blood agar, McConkey). Results were analyzed using SPSS software.
RESULTS: Staphylococcus aureus was the most offender and major organism causing contamination in all 4 groups of smokers and non-smokers with capped toothbrush and smokers and non-smokers with uncapped toothbrush. A correlation test was done using Pearson’s correlation, showing a positive correlation between uncapped non-smokers and capped smokers (P = 0.050).
CONCLUSION: The microbial contamination of capped and uncapped toothbrushes of adult smoker and non-smoker men was quite high and hence, it increases the risk of dental diseases.

Keywords

Main Subjects

References

  1. Nelson-Filho P, Isper AR, Assed S, Faria G, Ito IY. Effect of triclosan dentifrice on toothbrush contamination. Pediatr Dent 2004; 26(1): 11-6.
  2. Downes J, Hooper SJ, Wilson MJ, Wade WG. Prevotella histicola sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 2008; 58(Pt 8): 1788-91.
  3. Mehta A, Sequeira PS, Bhat G. Bacterial contamination and decontamination of toothbrushes after use. N Y State Dent J 2007; 73(3): 20-2.
  4. Obika I, Onuorah S. Bacterial contamination of used manual toothbrushes obtained from some students of Nnamdi Azikiwe. Univers J Microbiol Res 2015; 3(4): 56-9.
  5. Peker I, Akarslan Z, Basman A, Haciosmanoglu N. Knowledge and behavior of dentists in a dental school regarding toothbrush disinfection. Braz Oral Res 2015; 29: 48.
  6. Cobb CM. The Tooth Brush as a Cause of Repeated Infections of the Mouth. Boston Med Surg J 1920; 183(9): 263-4.
  7. Burki T. Thomas Frieden takes on the CDC. Lancet Infect Dis 2009; 9(7): 405.
  8. Frazelle M. Healthcare acquired infection risk and toothbrush contamination in the ICU [PhD Thesis]. Tampa, FL: University of South Florida; 2011.
  9. Sammons RL, Kaur D, Neal P. Bacterial survival and biofilm formation on conventional and antibacterial toothbrushes. Biofilms 2004; 1(2): 123-30.

10. Gautam B, Pokhrel S, Aryal S, Basnet A. Efficacy of toothpaste in reducing micro-flora isolated from toothbrush. Kathmandu University Journal of Science, Engineering and Technology 2017; 13(2): 71-8.

11. Iqbal S, Ahmed S, Aslam A, Kashif M, Khan I, Khan N. microbial flora on the white coats of dental staff, Karachi. Int JEndorsing Health Sci Res 2020; 8(1): 21-7.

12. Khan MI, Kashif M, Ehsan R, Faizuddin M, Iqbal S, Jamil H.  Microbial contamination of white coats, hands and mobile phones of health care workers. Int J Pathol 2020; 18(2): 58-62.

13. Naik R, Ahmed Mujib BR, Telagi N, Anil BS, Spoorthi BR. Contaminated tooth brushes-potential threat to oral and general health. J Family Med Prim Care 2015; 4(3): 444-8.

14. Dhifaf MS. Effectiveness of different cleanser solutions on the microbial contamination of toothbrushes. Journal of Kerbala University 2011; 7(1): 302-7.

15. Cheesbrough M. District laboratory practice in tropical countries. 2nd ed. Cambridge, UK: Cambridge University Press; 2006.

16. Kuboniwa M, Lamont RJ. Subgingival biofilm formation. Periodontol 2000 2010; 52(1): 38-52.

17. Mansoori N, Bakar I, Shahid N, Mubeen SM. Microbial contamination; a survey of microbial contamination of toothbrushes among general population of Karachi. Professional Med J 2018; 25(11):1785-90.

18. Petersen PE, Kandelman D, Arpin S, Ogawa H. Global oral health of older people--call for public health action. Community Dent Health 2010; 27(4 Suppl 2): 257-67.

19. Konidala U, Nuvvula S, Mohapatra A, Nirmala SV. Efficacy of various disinfectants on microbially contaminated toothbrushes due to brushing. Contemp Clin Dent 2011; 2(4): 302-7.

20. Raiyani CM, Arora R, Bhayya DP, Dogra S, Katageri AA, Singh V. Assessment of microbial contamination on twice a day used toothbrush head after 1-month and 3 months: An in vitro study. J Nat Sci Biol Med 2015; 6(Suppl 1): S44-S48.

21. Saini R, Kulkarni V. Toothbrush: A favorable media for bacterial growth. Int J Exp Dent Sci 2013; 2(1): 27-8.

22. Thamke MV, Beldar A, Thakkar P, Murkute S, Ranmare V, Hudwekar A. Comparison of bacterial contamination and antibacterial efficacy in bristles of charcoal toothbrushes versus noncharcoal toothbrushes: A microbiological study. Contemp Clin Dent 2018; 9(3): 463-7.

23. Talaat DM, Sharaf AAE, Ghoneim MAE, El-Shazly SA, El Meligy OAES. Efficacy of two mouth rinse sprays in inhibiting Streptococcus mutans growth on toothbrush bristles. Saudi Dent J 2018; 30(4): 365-72.

24. Karibasappa GN, Nagesh L, Sujatha BK. Assessment of microbial contamination of toothbrush head: An in vitro study. Indian J Dent Res 2011; 22(1): 2-5.

25. D'Silva I, Kothari T, Shah R, Sahetya S. Toothbrush disinfection - is your toothbrush making you sick? J Indian Dent Assoc 2007; 1: 88-91.

Journal of Oral Health and Oral Epidemiology
Volume 10, Issue 2
April 2021
Pages 100-106

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History

  • Receive Date: 16 March 2020
  • Revise Date: 22 July 2020
  • Accept Date: 07 October 2020

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