Evaluation of the effect of Kidi Lact probiotic product on streptococcus mutans in saliva in a group of 8-12-year-old children

Document Type : Original Article(s)

Authors

1 Associate Professor, Department of Pediatric Dentistry, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

2 Dentist, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

3 Professor, Kerman Social Determinants on Oral Health Research Center AND Oral and Dental Diseases Research Center AND Department of Pediatric Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran

4 PhD Candidate, Occupational Environment Research Center, Rafsanjan University of Medical Sciences, Rafsanjan AND Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran

5 Resident, Department of Pediatric Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran

Abstract

BACKGROUND AND AIM: Previous studies have shown that lactobacillus presented in food products affects oral ecology. In this study, we decided to show the effect of periodic use of Kidi Lact, a product containing probiotic bacteria which is recommended for improvement of gastrointestinal (GI) function in babies and children by physicians, on level of streptococcus mutans (S. mutans) in saliva among children between 8-12 years old. There are no previous studies in this relation.METHODS: This randomized crossover double-blind study was conducted on 30 healthy children between the age of 8-12 years. It included four steps. In the first step, children randomly were divided in two groups; in the step two, one of them used 1gr Kidi Lact every day and other group used placebo every day during two weeks, randomly. In the third step, children used none of them for one month, and finally in the step four, those who used Kidi Lact at step two, used placebo and other group vice versa. At the end of each step, samples of the children saliva was prepared and then, the number of S. mutans in the non-stimulated saliva samples was measured with microbial culture method. SPSS statistical software was used for data analysis.RESULTS: S. mutans reduction in saliva after using Kidi Lact was statistically significant (P < 0.001), but it showed no significant reduction after using placebo (P = 0.100). The amount of S. mutans in the two groups had no significant difference before using Kidi Lact and placebo (P = 0.406). Amount of S. mutans in the groups after using placebo and Kidi Lact was significant (P < 0.001).CONCLUSION: According to the present study, periodic use of Kidi Lact as a mouth wash has a strong effect on amount of S. mutans in saliva. So, physicians can be advised to tell their patients about rinsing of Kidi Lact before its swallowing.

Keywords

References

  1. Roberson T, Heymann HO, Swift EJ. ARABIC - Sturdevant's art and science of operative dentistry: Arabic bilingual edition. London, UK: Elsevier Health Sciences UK; 2013.
  2. Ahola AJ, Yli-Knuuttila H, Suomalainen T, Poussa T, Ahlstrom A, Meurman JH, et al. Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch Oral Biol 2002; 47(11): 799-804.
  3. Rugg-Gunn A. Dental caries: Strategies to control this preventable disease. Acta Med Acad 2013; 42(2): 117-30.
  4. Marshall TA, Levy SM, Broffitt B, Warren JJ, Eichenberger-Gilmore JM, Burns TL, et al. Dental caries and beverage consumption in young children. Pediatrics 2003; 112(3 Pt 1): e184-e191.
  5. Tantbirojn D, Huang A, Ericson MD, Poolthong S. Change in surface hardness of enamel by a cola drink and a CPP-ACP paste. J Dent 2008; 36(1): 74-9.
  6. Chuang LC, Huang CS, Ou-Yang LW, Lin SY. Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora. Clin Oral Investig 2011; 15(4): 471-6.
  7. Caglar E, Kavaloglu SC, Kuscu OO, Sandalli N, Holgerson PL, Twetman S. Effect of chewing gums containing xylitol or probiotic bacteria on salivary mutans streptococci and lactobacilli. Clin Oral Investig 2007; 11(4): 425-9.
  8. Poureslami H, Pishbin L, Eslaminejad Z, Jahani MF, Rashid FM. The effects of a dairy probiotic product, espar, on salivary calcium and mutans streptococci. J Dent Res Dent Clin Dent Prospects 2013; 7(3): 147-51.
  9. Cildir SK, Germec D, Sandalli N, Ozdemir FI, Arun T, Twetman S, et al. Reduction of salivary mutans streptococci in orthodontic patients during daily consumption of yoghurt containing probiotic bacteria. Eur J Orthod 2009; 31(4): 407-11.
  10. Moeiny P, Jamei N, Mohamadi M, SHafizadeh N, Valaei N, Rahbar M et al . Effect of a probiotic yogurt produced in Iran on the salivary counts of Streptococcus mutans. J Res Dent Sci 2013; 10(2): 73-82. [In Persian].
  11. Lee SH, Kim YJ. A comparative study of the effect of probiotics on cariogenic biofilm model for preventing dental caries. Arch Microbiol 2014; 196(8): 601-9.
  12. Caglar E, Kargul B, Tanboga I. Bacteriotherapy and probiotics' role on oral health. Oral Dis 2005; 11(3): 131-7.
  13. Yoo SY, Park SJ, Jeong DK, Kim KW, Lim SH, Lee SH, et al. Isolation and characterization of the mutans streptococci from the dental plaques in Koreans. J Microbiol 2007; 45(3): 246-55.
  14. Caglar E, Sandalli N, Twetman S, Kavaloglu S, Ergeneli S, Selvi S. Effect of yogurt with Bifidobacterium DN-173 010 on salivary mutans streptococci and lactobacilli in young adults. Acta Odontol Scand 2005; 63(6): 317-20.
  15. Caglar E, Kuscu OO, Selvi KS, Kavaloglu CS, Sandalli N, Twetman S. Short-term effect of ice-cream containing Bifidobacterium lactis Bb-12 on the number of salivary mutans streptococci and lactobacilli. Acta Odontol Scand 2008; 66(3): 154-8.
  16. Caglar E, Cildir SK, Ergeneli S, Sandalli N, Twetman S. Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol Scand 2006; 64(5): 314-8.
  17. Petti S, Tarsitani G, D'Arca AS. A randomized clinical trial of the effect of yoghurt on the human salivary microflora. Arch Oral Biol 2001; 46(8): 705-12.
  18. Nase L, Hatakka K, Savilahti E, Saxelin M, Ponka A, Poussa T, et al. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res 2001; 35(6): 412-20.
  19. Nikawa H, Makihira S, Fukushima H, Nishimura H, Ozaki Y, Ishida K, et al. Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. Int J Food Microbiol 2004; 95(2): 219-23.
  20. Lesan S, Rahbar M, Valaei N, Abbasi Vardoogh S, Abbasi Vardoogh M. Effect of probiotic yoghurt on salivary Streptococcus mutansand Lactobacillus. Pajouhesh Dar Pezeshki 2013; 37(3): 151-7. [In Persian].
Journal of Oral Health and Oral Epidemiology
Volume 7, Issue 4 - Serial Number 4
130
October 2018
Pages 205-210

Files

History

  • Receive Date: 08 January 2019
  • Revise Date: 24 April 2024
  • Accept Date: 12 March 2019

Share

How to cite

Statistics