Comparing salivary level of alpha-amylase in patients with recurrent aphthous stomatitis and healthy individuals

Document Type : Original Article

Authors

1 Associate Professor, Oral and Dental Disease Research Center AND Department of Oral Medicine, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran

2 Professor, Cellular and Molecular Research Center AND Department of Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

3 Dentist, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran

Abstract

BACKGROUND AND AIM: Recurrent aphthous stomatitis (RAS) results due to a multiple of causes, amongst which stress is one of the most important factors. On the other hand, salivary alpha (α)-amylase (SAA) is a secretory protein that increases in stress conditions. This study evaluated SAA level in subjects with RAS.
METHODS: In this case-control (descriptive-analytical) study, unstimulated saliva samples were collected from
27 patients with RAS and 29 healthy controls. SAA activity was determined by spectrophotometric method using commercially available kit according to manufacturer procedure. Data were analyzed using SPSS software with t test
(P < 0.05 was considered significant).
RESULTS: SAA level in patients with RAS was 80.78 ± 4.69 U/ml and 65.61 ± 27.52 U/ml during recurrence and recovery, respectively (P = 0.005). SAA level in control group was 19.99 ± 4.65 U/ml. There was a significant difference in the SAA level between RAS and control groups.
CONCLUSION: SAA level has been increased in patients with aphthous ulcer during recurrence, which may indicate an association between aphthous ulcer and stress.

Keywords

References

  1. Glick M. Burket's oral medicine. 12th ed. Shelton, CT: People's Medical Publishing House; 2014. p. 73-5.
  2. Chiang CP, Yu-Fong CJ, Wang YP, Wu YH, Wu YC, Sun A. Recurrent aphthous stomatitis - Etiology, serum autoantibodies, anemia, hematinic deficiencies, and management. J Formos Med Assoc 2019; 118(9): 1279-89.
  3. Nadendla LK, Meduri V, Paramkusam G, Pachava KR. Relationship of salivary cortisol and anxiety in recurrent aphthous stomatitis. Indian J Endocrinol Metab 2015; 19(1): 56-9.
  4. Vineetha R, Pai KM, Vengal M, Gopalakrishna K, Narayanakurup D. Usefulness of salivary alpha amylase as a biomarker of chronic stress and stress related oral mucosal changes - a pilot study. J Clin Exp Dent 2014; 6(2): e132-e137.
  5. Nater UM, Rohleder N, Gaab J, Berger S, Jud A, Kirschbaum C, et al. Human salivary alpha-amylase reactivity in a psychosocial stress paradigm. Int J Psychophysiol 2005; 55(3): 333-42.
  6. Preeti L, Magesh K, Rajkumar K, Karthik R. Recurrent aphthous stomatitis. J Oral Maxillofac Pathol 2011; 15(3): 252-6.
  7. Shirasaki S, Fujii H, Takahashi M, Sato T, Ebina M, Noto Y, et al. Correlation between salivary alpha-amylase activity and pain scale in patients with chronic pain. Reg Anesth Pain Med 2007; 32(2): 120-3.
  8. Tzira D, Prezerakou A, Papadatos I, Vintila A, Bartzeliotou A, Apostolakou F, et al. Salivary biomarkers may measure stress responses in critically ill children. SAGE Open Med 2018; 6: 2050312118802452.
  9. Takai N, Yamaguchi M, Aragaki T, Eto K, Uchihashi K, Nishikawa Y. Effect of psychological stress on the salivary cortisol and amylase levels in healthy young adults. Arch Oral Biol 2004; 49(12): 963-8.
  10. Rashkova MR, Ribagin LS, Toneva NG. Correlation between salivary alpha-amylase and stress-related anxiety. Folia Med (Plovdiv) 2012; 54(2): 46-51.
  11. Gallo CB, Mimura MA, Sugaya NN. Psychological stress and recurrent aphthous stomatitis. Clinics (Sao Paulo) 2009; 64(7): 645-8.
  12. Kunikullaya UK, Kumar MA, Ananthakrishnan V, Jaisri G. Stress as a Cause of Recurrent Aphthous Stomatitis and Its Correlation with Salivary Stress Markers. Chin J Physiol 2017; 60(4): 226-30.
Journal of Oral Health and Oral Epidemiology
Volume 8, Issue 4
November 2019
Pages 173-176

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History

  • Receive Date: 04 August 2019
  • Revise Date: 11 November 2019
  • Accept Date: 15 December 2019

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