Comparison of salivary and serum homocysteine levels in oral squamous cell carcinoma

Document Type : Original Article

Authors

1 Department of Oral and Maxillofacial Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Restorative Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

3 Oncology Surgeon, Tabriz, Iran

4 Department of Prosthodontics, Tabriz University of Medical Sciences, Tabriz, Iran

5 Department of Community Oral Health, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: No factor alone has been defined as the cause of oral squamous cell carcinoma (SCC). Identification of biochemical markers involved in SCC metabolic reactions is important in SCC diagnosis. Homocysteine is an amino acid whose altered levels have been observed in various malignancies. This study aimed to assess the serum and salivary levels of homocysteine in patients with SCC and compare these data with those of healthy individuals.
Methods: In this case-control analytical study, 21 patients with oral SCC and 21 healthy subjects were studied. Salivary and serum samples were obtained, and homocysteine levels were evaluated with an HCY ELISA Kit. Independent t-test and Pearson correlation coefficient were used, and statistical analysis was done using SPSS 17. A P value < 0. 05 was considered significant.
Results: The level of serum homocysteine was 3.71 ± 2.5 in the patient group and 2.01 ± 2.11 in the control group (P = 0.008). The salivary homocysteine levels were 3.12 ± 1.66 in the case group and 2.93 ± 1.71 in control group (P = 0.782).
Conclusion: Homocysteine levels in the serum might be a good marker for diagnosis of oral SCC; however, more research is needed on using salivary homocysteine levels as a diagnostic indicator.

Keywords

References

  1. Dumache R. Early diagnosis of oral squamous cell carcinoma by salivary microRNAs. Clin Lab. 2017;63(11):1771-6. doi:10.7754/Clin.Lab.2017.170607.
  2. Ibrahim SA, Ahmed ANA, Elsersy HA, Darahem IMH. Elective neck dissection in T1/T2 oral squamous cell carcinoma with N0 neck: essential or not? A systematic review and metaanalysis. Eur Arch Otorhinolaryngol. 2020;277(6):1741-52. doi:10.1007/s00405-020-05866-3.
  3. Rivera C, Oliveira AK, Costa RAP, De Rossi T, Paes Leme AF. Prognostic biomarkers in oral squamous cell carcinoma: a systematic review. Oral Oncol. 2017;72:38-47. doi:10.1016/j.oraloncology.2017.07.003.
  4. Rao RS, Chatura KR, Sv S, Prasad K, Lakshminarayana S, Ali FM, et al. Procedures and pitfalls in incisional biopsies of oral squamous cell carcinoma with respect to histopathological diagnosis. Dis Mon. 2020;66(12):101035. doi:10.1016/j.disamonth.2020.101035.
  5. Sargeran K, Murtomaa H, Safavi SM, Vehkalahti M, Teronen O. Malignant oral tumors in Iran: ten-year analysis on patient and tumor characteristics of 1042 patients in Tehran. J Craniofac Surg. 2006;17(6):1230-3. doi:10.1097/01.scs.0000246728.23483.ce.
  6. Persichilli S, Gervasoni J, Iavarone F, Zuppi C, Zappacosta B. A simplified method for the determination of total homocysteine in plasma by electrospray tandem mass spectrometry. J Sep Sci. 2010;33(20):3119-24. doi:10.1002/jssc.201000399.
  7. Colovic MB, Vasic VM, Djuric DM, Krstic DZ. Sulphurcontaining amino acids: protective role against free radicals and heavy metals. Curr Med Chem. 2018;25(3):324-35. doi:10.2174/0929867324666170609075434.
  8. Feller L, Lemmer J. Oral squamous cell carcinoma: epidemiology, clinical presentation and treatment. J Cancer Ther. 2012;3(4):263-8. doi:10.4236/jct.2012.34037.
  9. Zhang D, Wen X, Wu W, Guo Y, Cui W. Elevated homocysteine level and folate deficiency associated with increased overall risk of carcinogenesis: meta-analysis of 83 case-control studies involving 35,758 individuals. PLoS One. 2015;10(5):e0123423. doi:10.1371/journal.pone.0123423.
  10. Weinstein SJ, Gridley G, Harty LC, Diehl SR, Brown LM, Winn DM, et al. Folate intake, serum homocysteine and methylenetetrahydrofolate reductase (MTHFR) C677T genotype are not associated with oral cancer risk in Puerto Rico. J Nutr. 2002;132(4):762-7. doi:10.1093/jn/132.4.762.
  11. Nacci A, Dallan I, Bruschini L, Traino AC, Panicucci E, Bruschini P, et al. Plasma homocysteine, folate, and vitamin B12 levels in patients with laryngeal cancer. Arch Otolaryngol Head Neck Surg. 2008;134(12):1328-33. doi:10.1001/archotol.134.12.1328.
  12. Jaganath SS, Kaveri H, Okade A. Determination of plasma homocysteine levels in oral submucous fibrosis & oral squamous cell carcinoma using high performance liquid chromatography and its plausibility as a potential biomarker. World J Pharm Res. 2016;5(4):1125-41. doi:10.20959/wjpr20164-5907.
  13. Narang D, Shishodiya S, Sur J, Fatma N. Estimation of serum homocysteine: as a diagnostic marker of oral sub mucous fibrosis. J Carcinog Mutagen 2014;5(5):187. doi:10.4172/2157-2518.1000187.
  14. Erugula SR, Kandukuri MK, Danappanavar PM, Ealla KK, Velidandla S, Manikya S. Clinical utility of serum homocysteine and folate as tumor markers in oral squamous cell carcinoma- a cross-sectional study. J Clin Diagn Res. 2016;10(10):ZC24-ZC8. doi:10.7860/jcdr/2016/19656.8637.
  15. Lorch JH, Posner MR, Wirth LJ, Haddad RI. Seeking alternative biological therapies: the future of targeted molecular treatment. Oral Oncol. 2009;45(4-5):447-53. doi:10.1016/j.oraloncology.2008.08.009.
  16. Chang H, Ma M, Ma R, Zhang C, Zeng W, Xing LQ. Folate deficiency and aberrant expression of cell adhesion molecule 1 are potential indicators of prognosis in laryngeal squamous cell carcinoma. Oncol Lett. 2016;12(6):4510-4. doi:10.3892/ol.2016.5264.
  17. Kabzinski J, Maczynska M, Majsterek I. MicroRNA as a novel biomarker in the diagnosis of head and neck cancer. Biomolecules. 2021;11(6):844. doi:10.3390/biom11060844.
  18. Kilic N, Dagli N, Aydin S, Erman F, Bek Y, Akin O, et al. Saliva/serum ghrelin, obestatin and homocysteine levels in patients with ischaemic heart disease. Cardiovasc J Afr. 2017;28(3):159-64. doi:10.5830/cvja-2016-075.
  19. Hasan T, Arora R, Bansal AK, Bhattacharya R, Sharma GS, Singh LR. Disturbed homocysteine metabolism is associated with cancer. Exp Mol Med. 2019;51(2):1-13. doi:10.1038/s12276-019-0216-4.
  20. Almadori G, Bussu F, Galli J, Cadoni G, Zappacosta B, Persichilli S, et al. Serum folate and homocysteine levels in head and neck squamous cell carcinoma. Cancer. 2002;94(4):1006-11. doi:10.1002/cncr.10343.
  21. Gorgulu O, Selcuk T, Ozdemir S, Sayar C, Beyazit Y, Akbas Y. Evaluation of the roles of serum vitamin B12, folate and homocysteine levels in laryngeal squamous cell carcinoma. J Int Med Res. 2010;38(6):2047-52. doi:10.1177/147323001003800619.
  22. Shiao SPK, Lie A, Yu CH. Meta-analysis of homocysteinerelated factors on the risk of colorectal cancer. Oncotarget. 2018;9(39):25681-97. doi:10.18632/oncotarget.25355.
Journal of Oral Health and Oral Epidemiology
Volume 12, Issue 1
March 2023
Pages 38-41

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History

  • Receive Date: 13 August 2021
  • Revise Date: 24 March 2022
  • Accept Date: 21 May 2022

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