A modification of a previous model for inflammatory tooth pain: Effects of different capsaicin and formalin concentrations and ibuprofen

Raoof, Maryam; Abbasnejad, Mehdi; Amirkhosravi, Ladan; Ebrahimnejad, Hamed; Raoof, Ramin

A modification of a previous model for inflammatory tooth pain: Effects of different capsaicin and formalin concentrations and ibuprofen

Document Type : Original Article(s)

Authors

Maryam Raoof ¹

Mehdi Abbasnejad ²

Ladan Amirkhosravi ³

Hamed Ebrahimnejad ³

Ramin Raoof ⁴

¹ Assistant Professor, Neuroscience Research Center and Department of Endodontics, Kerman University of Medical Sciences, Kerman, Iran

² Associate Professor, Department of Biology, School of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

³ Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran

⁴ MSc student, Department of Biology, School of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

BACKGROUND: This study aimed to solve the problems faced with the previous model of inflammatory tooth pain in rats. METHODS: After cutting 2 mm of the distal extremities, the polyethylene crowns were placed on the mandibular incisors. In contrast to the original model, we used flow composite instead of wire in order to maximize the retention of crowns. Different concentrations of capsaicin (10, 25 and 100 mg/ml) and formalin were administrated into the cavities under the crowns. The algesic agent-induced behaviors were evaluated. RESULTS: The modified model had no liquid leakage. Furthermore, composite allowed the crowns to remain for a longer period of time. Capsaicin 25, 100 mg/ml and formalin applications induced significantly more painful stimulation compared with control groups (P < 0.001). These responses were significantly reduced by the administration of ibuprofen, 20 minutes prior to the capsaicin 100 mg/ml injection. CONCLUSIONS: This model seems to be adequate for long-term pain related experiments in which fluid leakage elimination is important.

Keywords

Odontalgia

capsaicin

formalin

model

Rat