Evaluation of teeth whitening with application of novel toothpaste ‎containing ozone

Document Type : Original Article(s)


1 Associate Professor, Dental Materials Research Center and Department of Operative Dentistry, ‎School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

2 General Dentist, Department of Operative Dentistry, School of Dentistry, Mashhad University of ‎Medical Sciences, Mashhad, Iran

3 Associate Professor, Department of Medicinal Chemistry, School of Pharmacy, Mashhad ‎University of Medical Sciences, Mashhad, Iran

4 High School Student, Emam Hussein Institute, Mashhad, Iran‎


BACKGROUND AND AIM: The aim of this study was to evaluate the effectiveness of different whitening toothpastes, focusing on the experimental ozonated toothpaste. MATERIALS AND METHODS: This laboratory study included a sample of 48 sound human molar teeth. Teeth were randomly assigned into four groups according to toothpaste treatment to be evaluated objectively (colorimetric method) and subjectively (visual assessment) (n = 12). Group I: Ozonated toothpaste (experimental); Group II: Non-ozonated toothpaste (experimental); Group III: Aqua fresh whitening triple protection; and Group IV: Nasim (toothpaste without a chemical whitening agent). After tea staining and color assessment, the teeth were subjected to a tooth brushing regime as for 6 weeks, done twice a day, 2 min each time (total: 168 min). Next, color changes were determined after brushing by instrumental and visual methods. RESULTS: Analysis of variance and the Tukey tests were used for evaluating statistical data (α = 0.05). Color change by instrumental index showed that ozonated and aquafresh toothpastes increased teeth whitening; however, the amount color change was not significant (P > 0.050). With visual assessment there was a significant difference between mean color change among the four groups (P = 0.008). CONCLUSIONS: Ozonated toothpaste caused significant whitening changes in discolored teeth from a clinical point of view by visual assessment.